Journal: PLoS ONE

Article Title: Antirheumatic treatment is associated with reduced serum Syndecan-1 in Rheumatoid Arthritis

doi: 10.1371/journal.pone.0253247

Figure Lengend Snippet: Boxplot showing levels of syndecan-1, MMP-9 and TIMP-1 at baseline and after 6 weeks of antirheumatic treatment. Midline represents median. Bottom and top of the box represent 25 and 75 percentile and whiskers represent minimum and maximum values.

Article Snippet: Serum was analyzed in blinded fashion, using ELISA kits for detecting human syndecan-1 (Diaclone, 950.640.192), MMP-9 (R&D, DY911) and TIMP-1 (R&D, DY970), according to the manufacturer’s instructions.

Techniques:

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: Human TIMP-1 DuoSet ELISA , R&D Systems , Cat #DY970.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry

Journal: Arthritis Research & Therapy

Article Title: Interferon-γ inhibits interleukin-1β-induced matrix metalloproteinase production by synovial fibroblasts and protects articular cartilage in early arthritis

doi: 10.1186/ar2960

Figure Lengend Snippet: IFN-γ does not affect tissue protective TIMP-1 secretion by RA FLS in vitro . Fibroblast-like synoviocytes (FLS) were cultured from synovial tissue specimens obtained from rheumatoid arthritis (RA) patients at synovectomy or joint replacement. Eight cell lines were used at fourth passage. Cells were incubated in culture medium or in medium containing escalating doses of IFN-γ (0.1 to 10 ng/ml), IL-1β (0.1 ng/ml) or combinations of the two cytokines for 72 hours. TIMP-1 levels were quantified by ELISA in culture supernatants at 24, 48 and 72 hours; mean ± SEM TIMP-1 concentrations are reported. (A) TIMP-1 levels were significantly increased by IL-1β but not escalating concentrations of IFN-γ. IFN-γ did not affect IL-1β induced TIMP-1 production by RA FLS; (B) represents data obtained for 10 ng/ml IFN-γ ± IL-1β (0.1 ng/ml). Tukey's test was used in conjunction with a one-way analysis of variance to analyze differences in TIMP-1 levels. P values less than 0.05 were considered statistically significant. NS denotes non-significant change, * P < 0.05 and ** P < 0.005.

Article Snippet: MMP-3 was measured using the specific human BioSource CytoSet™ CHC1544 ELISA kit (Life Technologies Corporation, Carlsbad, California, USA), MMP-13 using the human Quantikine ® DM1300 ELISA kit (R&D Systems Europe, Ltd. Abingdon, U.K) and TIMP-1 using the human DuoSet ® DY970 ELISA kit (R&D Systems Europe, Ltd. Abingdon, U.K).

Techniques: In Vitro, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay